The tubes were then chilled until the oil sample was solidified prior to placing them in a test tube and held in a beaker of water equilibrated at 10 c for 16 h in a thermostat. Estimation of iodine value of fats and oils theory. Next add 20 ml of the potassium iodide solution and 100150 ml of deionised water. Manual on oils and fats food safety and standards authority. Determination of iodine value of oil lipids laboratory.
Close the erlenmeyer flask with a stopper, shakemix the solution for some seconds and let the sample stay in darkness for 5 minutes. Analogous to this procedure, other anhydrides can be converted into their corresponding acids. In a typical procedure the acid is treated with an excess of wijs iodine solution which a solution of iodine monochloride icl in glacial acetic acid. Feb 06, 2012 the aocs recommended method d195997 wijs procedure is broadly used for determination of iv. Specimen collection and submission instructions covid19. Collaborative test of determination of iodine value in fish oils. Wijs solution for determination of the iodine number cicl 0. Dilute 10 ml to 100 ml with water in a volumetric flask. Metal dishes 7 8 cm diameter and 2 3 cm deep provided with tight fitting slip on covers.
Title communication of sample analysis results for food. Compare the written policy procedure to what you actually observe in the laboratory to ensure the written policy procedure accurately reflects laboratory practice. The vegetable oil sample is reacted with an excess of iodine monochloride. Triglycerides are made up of three fatty acids linked to glycerol by fatty acyl esters. Its purpose is to carry out the operation correctly and always in. Determination of iodine content iodine titration free. Palmbased standard reference materials for iodine value and. Then 25 ml of wijs reagent was added to the flask using a safety pipette influenced chamber. Where l indicates the degree of lightness or darkness of the sample extended from 0 black to 100 white. The clay sample was taken from ibeshe deposit, epe road, ikorodu, lagos. For samples collected outside the laboratory, other informa2on important to the sample should be included on the label loca2on where sample was collected coordinates, address, etc. Guidelines for a physics lab reports a laboratory report has three main functions. The most popular procedure is known as the wijs method, but all methods suppose that a normal carboncarbon double bond takes up two atoms of iodine.
The iodine value or iodine adsorption value or iodine number or iodine index in chemistry is the mass of iodine in grams that is consumed by 100 grams of a chemical substance. Iodine is then introduced as an indicator for the presence of starch. In this procedure, the biodiesel sample reacts with wijs solution in the presence of a catalyst and. Determination of iodine number of palm oil wijscyclohexane. Iodine value is the average unsaturation measurement in fat sample.
Making laboratory reagents for general use salt lake metals. Determination of ffa and iodine value campuscrosswalk. Accurately weigh 510 g of sample into a dried, tared crucible. The colour were determined by hunter lab digital colorimeter model d25m, hunter associates labrotory, reston, va. About 5 g of accurately weighed povidoneiodine is placed in a 400ml beaker, and 200 ml of distilled water is added. Accepted 11766 revised 41194 iodine number wijs method principle the vegetable oil sample is reacted with an excess of iodine monochloride solution wijs. Pyridinefree karl fischer reagents are available from lab. Characterization, acid activation, and bleaching performance. The iodine concentration is then determined by titration with sodium thiosulfate. Due to the physical nature of the matrix, sample preparation for solids requires the most attention, and therefore is discussed at great length section 12. Draw 1 ml of the solution in test tube a with the pipette, transfer it to test tube b and mix the contents of test tube.
Emergency procedures indicate how spills, personnel exposureinjury, and other accidents should be handled and by whom. The tip of the flask is moistened with potassium iodide. Analogous to this procedure, other anhydrides can be converted into their. Wijs special solution for iodine numberanalyst 58, 5237, 1933. Customer reference sampling procedure sample collected by sl. Standard test method for determination of iodine value of.
Handling and storage protect against physical damage. Determination of iodine content free download as powerpoint presentation. The reagent blank was also performed by using isooctane instead of sample solution. Observe laboratory practices look at what the laboratory is actually doing. For sample weigh the recommended amount of oil see working range. It is yet difficult to quantitatively hypothesize the iodine values of the oils. Fill test tube a with 10 ml of the solution and fill test tube b with 9 ml of a buffer to dilute the original solution. Wijs solution lab equipment lab supplies spectrum chemical. In our experience, it is an important and easily available parameter in fat selection. Why is a starch added at the end of the reaction of.
The analytical procedure can be performed even without a laboratory hood as no. Send both blood samples with a properlycompleted clinical chemistry request form requesting ogtt or glucose tolerance test to the pathology laboratory. Nh4oh, allow to stand over night and filter if a precipitate has formed. Where, iodine value was calculated by using the formula. One example of a recipe is to dissolve 2 grams of diiodine. Slac national accelerator laboratory environment, safety, health, and quality division chapter 53 laboratory standard operating procedure template 20 may 20 slaci7300a09j009r000 5 of 6 10. Iodine number is a measure of the total number of double bonds present in fats and oils. Preparing a policy or procedure document for uc santa cruz infoslug online policy and procedure system is not as mysterious or difficult as you might think. Next, the tallow is reacted with an excess solution of iodine monochloride in glacial acetic acid also know as wijs solution for 30 minutes. Please consider analysis environment, external factors and sample nature for optimal conditions before the. Add the recommended volume of titration solvent 20 or 25 ml. In linoleic acid and cod liver oil the hanus method gives 2 to 4 per cent higher values than the other. Pdf a new and fast method was developed to determine the iodine number of biodiesel.
Petroleum iodine value of oil and fat kyoto electronics. The coconut meat was homogenously dried and prevented from scorching of the meat through frequent turning of the shredded meat. Carbon tetrachloride is a carcinogen in laboratory animals. Determination of iodine value of fats and oil discover. Merck kgaa, darmstadt, germany is accredited as calibration laboratory. Sample collection procedures sample handling procedures evaluation of completeness recordkeeping and reporting procedures a thorough sampling plan documents the procedures employed to preserve and protect the material collected during the sampling event while it is being transported from the field to the laboratory. About 2 grams of the oil was weighed, in a small beaker and dissolved in 50 ml of chloroform, transferred into a hundred volumetric flask and diluted to the mark with chloroform. This guide is designed to explain the campus policy and procedure framework, to help policy and procedure owners organize their written documentation, and to act as a resource. Pdf fast determination of iodine number of biodiesel using. Physicochemical properties of virgin coconut oil extracted. One of the first few basic science experiments conducted is to add iodine to potato slices and watch it turn blue black.
It is a detailed, written instruction used to achieve uniformity in the performance of specific function. To determine the iodine value of fats and oils and thus estimate the unsaturation of the fats and oils. The total iodine value also includes the iodide ions which are necessary for formation of the complex. If the procedure uses a conical flask with a ground glass stopper, it is possible to pour the solution into a beaker just before the titration, using deionised water to rinse the flask. Writing, issuing and retrieving standard operating procedures revision. The buffer is typically water, but it also can be other liquids, depending on the solution in test tube a.
Mix well and allow to stand in dark for exactly 30min with occasional shaking. Determination of iodine value with a fourier transformnear. Iodine numbers are often used to determine the amount of unsaturation in fatty acids. The composition of unsaturated fats in olive oil, coconut oil, canola oil and peanut oil are 86%, 9%, 93%, 80%. If a policy or procedure requires a change, a new policy or procedure will be written, approved by the laboratory director, and distributed to. Guidelines for a physics lab reports baylor university. Note if practice deviates from the documented policies procedures. Fatty acid composition of coconut oil and some other vegetable oils v egetable oils c8. Manual for analysis of oils and fats oils and fats are important parts of human diet and more than 90 per cent of the world production from vegetable, animal and marine sources is used as food or as an ingredient in food products. For blank determination, prepare a solution in the same way as indicated below but without a sample. Moreover, a standard operating procedure document prevents employers. This solvent is used to allow for the reaction to take place. Nwfhs laboratory procedures manual second edition, june 2004 chapter 3 page 2 i. Simple spectrophotometric method for determination of.
Procedure title procedure author date of creation revision name of responsible person principal investigator, laboratory supervisor, or autonomous researcher location to be performed building or lab number, beam line proposal numbers. Degree of unsaturation of different oils schoolworkhelper. Add 20 ml 10 ml can sometimes be sufficient of wijs solution see wijs solution and burette. The analytical results obtained with this procedure were compared with those from wijs method. These procedure templates communicate what needs to be done on a routine basis. The method involves adding halogen in the presence of potassium iodide and the titrating the released iodine with sodium thiosulfate using a standard starch solution as the indicator. Estimation of iodine value of fats and oils procedure. Iodine value iv is the percentage of its own weightwith which a fat will combineof halogen calculated as iodine, the conditions of the test being indicated. Determination of the iodine value of oils and fats results. The sample was prepared in triplicate and kept refrigerated afterwards until further use.
Iodine value of edible oils and fats according to wijs aoac. Genstat 5 reference manual, clarendon press, oxford, 1988. Cml test method, reference number tests on soils and gravels 1. Raw materials laboratory testing and documentation author. Aphl laboratory internal audit plan 7 schedule this section should give an overview of the timetable for the audits. A standard operating procedure sop is the document which describes regularly recurring operations relevant to the quality of investigations. Wijs reagent was carried out in a similar fashion omitting the sample.
Sample received on analysis started on analysis completed on 05. Vegetable oils determining degree of unsaturation and. Study of performance test of ruston diesel engine using. Insert immediately the cuvette into the reading cell identified by green led. Change record this should be a list or table summarizing the main updates and changes embodied in each version of the protocol and where appropriate, the reasons for these. Extraction and physicochemical characterization of seed oil. Add 25ml of hanus iodine solution using a pipette, draining it in a definite time. The iodine value is in direct proportion to the number of mono and polyunsaturated bonds within the constituent fatty acids.
Iodine monochloride, solution, dwk life sciences kimble kimax iodine determination flasks with ptfe stopper, iodine. The analytical procedure can be performed even without a laboratory hood as no iodine vapors are released if the method is done as indicated. Guidelines on the organiza2on of samples in a laboratory. Transfer 25 ml of cc14 and 25 ml of wijs solution each of two500 ml iodine titration flasks. This unsaturation is in the form of double bonds, which react with iodine compounds.
To understand the flow of raw material through the laboratory, testing and the documentation of the test data. It is expressed as the iodine in grams that will react with the double bonds in 100 grams of fats or oils. Oils and fats are a rich source of dietary energy and contain more than twice the caloric value of equivalent amount of sugar. Add 20ml of iodine monochloride reagent in to the flask. For the determination of the iodine number of oleic and ricin oleic acids both the hanus and the rosenmund methods give the same value which is very near the theoretical. Fast determination of iodine number of biodiesel using capillary. In this procedure, the biodiesel sample reacts with wijs solution in the. Fssai parameters acid value iodine value wijs method. Life without oil is not possible, especially in cooking. Hence, a consistent highquality output can be guaranteed from every employee. Sampling and preparation for laboratory measurements measurements for performing a survey or deciding that sampling methods followed by laboratory analysis are necessary. Washington state phl, waphl, public health lab, laboratory tests, lab specimen, cultures, diagnostic, reference, doh publication 301020 created date 252020 2. Standard operating procedures, or commonly known as sops, are the forefront documents presented to every employee.
Iv is obtained by firstly dissolving a weighed sample of tallow in a solvent such a cyclohexane and glacial acetic acid. The flask is gently rotated to mix the contents thoroughly. Accepted 11766 revised 41194 iodine number wijs method principle the vegetable oil sample is reacted with an excess of iodine monochloride solution wijs reagent under controlled conditions. Strain the oil sample through a filter paper to remove all the solid material and moisture present in the sample. Pipette out 10ml of fat sample dissolved in chloroform to an iodination flask labeled as test. Quality assessment of mustard oil in deep fat frying. It is then dissolved in about 10ml of carbon tetrachloride. An internal audit is a formal laboratory activity that must be performed in accordance with a documented procedure and on a regular schedule. Prepare the following solutions starch solution 10% potassium iodide solution 0. The first step in standardizing the primary process is the documentation of the procedures of all the tests currently performed in the laboratory in standard operating procedures sops. Unfortunately, this virtual lab requires adobe flash player. Corn oil analysis h321 analytical methods of the member companies of the corn refiners association, inc. This standard operating procedure sop is for a specific laboratory procedure or experiment.
The crude palm oil was obtained locally from the yaba market. What develop sops for all the tests identified in the list made earlier in phase 1. To 200 ml of glacial acetic acid that will pass the dichromate test for reducible matter, add 12 g of dichloroamine t paratoluenesulfonedichloroamide, and 16. The procedure is applicable to fats and oils from corn, grain sorghum and other vegetable.
Other materials used were hydrochloric acid, acetic acid, caustic soda, wijs reagent, sodium thiosulphate all analytical grades reagents, distilled water, and phenolphthalein. In order to strictly compare instrumental procedures potentiometry was also applied to the wijs procedure. First sample for the buyer second sample for the seller third sample for final reference, if necessary. At least three clean capillary tubes were initially dipped into a completely melted oil sample to a depth of 10 mm.
The testing of raw materials provides assurance that quality materials are used in production. The determination is carried out by dissolving a weighed sample in a nonpolar solvent such as cyclohexane, then adding glacial acetic acid. Iodine value of edible oils and fats according to wijs. Fatty acids are long chain hydrocarbons with carboxyl groups cooh groups. Selection and use of personal protective equipment and attire in laboratories this procedure describes how to implement yale universitys university personal protective equipment policy policy in a laboratory. Freshdry process the white meat of the coconut was shredded and dried in an aerated oven at 350c for approximately 48 h. Jeev goodness cold pressed pure gingelly oil altmay18f20767 verbal work order customer parameters fssai parameters acid value iodine value wijs method. A green potentiometric method for the determination of the. However, a rough order of increasing degree of unsaturation can be hypothesized by examining the composition of fatty acids in the oils. Proximate analysis and physicochemical properties of. Introduction laboratory tracking is an important component of quality control because it ensures proper sample identification and accuracy in reported test results. Store in a cool, dry wellventilated location, away from any area where the fire hazard may be acute.
This method provides an assay value for a sample of povidoneiodine. Scribd is the worlds largest social reading and publishing site. The iodine value for native oilsfats of defined plant origin is easily accessible from tables or from the fat supplier table 3. Take the specimens for the individual tests from this. A green potentiometric method for the determination of the iodine.
Procedure the system of drawing of samples tendered for delivery will be as prescribed in the bureau of indian standards procedure. Label as specified by the protocol and included the sample date and time or 2 hour. Background a identify previous research on the topic. Sep 22, 2008 smp was measured using the following procedure. Sample substance chemicals titrant instruments results chemistry accessories indication dm140sc at sensor 2 iodine value of edible oils and fats according to wijs aoac method 1 dissolve the sample in ccl 4, add 25 ml wijs solution and keep in the dark for approx. Who laboratory manual for the examination and processing.
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